Nutrient Agar

White powder, free-flowing, homogeneous, 5.0% solution, soluble in purified water upon boiling. Solution is colorless. pH 7.2

Cultural Response

Prepare the medium per label directions.

Staphylococcus aureu	Colorless colony, colony diameter 1.5-1.7 mm.
Streptococcus pyogenes	Colorless colony, colony diameter is like needl peak.
Shigella dysenteriae	Colorless colony, colony diameter 1.5-2.0 mm.
Salmonella sp	Colorless colony, colony diameter 1.8-2.0 mm.
Pseudomonas aeruginosa	Colorless colony, colony diameter 2.5-3.0 mm.
Escherichia  coli	Colorless colony, colony diameter 2.3 mm.

Formulae

Approximate Formula* Per Liter

                          Peptone ....................................................... 10.0 g

                          Beef extract ................................................... 3.0 g

                          Sodium Chloride ............................................ 5.0 g

                          Agar ............................................................. 20.0 g

Usage

Common bacterial culture medium, also used in blood agar base.

Nutrient Agar